5-Exo-hydroxycamphor dehydrogenase explained

5-exo-hydroxycamphor dehydrogenase
Ec Number:	1.1.1.327

5-exo-hydroxycamphor dehydrogenase (F-dehydrogenase, FdeH) is an enzyme with systematic name 5-exo-hydroxycamphor:NAD⁺ oxidoreductase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

5-exo-hydroxycamphor + NAD⁺

\rightleftharpoons bornane-2,5-dione + NADH + H⁺

This enzyme contains Zn²⁺. It is isolated from Pseudomonas putida.

Notes and References

1. Rheinwald JG, Chakrabarty AM, Gunsalus IC . A transmissible plasmid controlling camphor oxidation in Pseudomonas putida . Proceedings of the National Academy of Sciences of the United States of America . 70 . 3 . 885–9 . March 1973 . 4351810 . 433381 . 10.1073/pnas.70.3.885 . 1973PNAS...70..885R . free .
2. Koga H, Yamaguchi E, Matsunaga K, Aramaki H, Horiuchi T . Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida . Journal of Biochemistry . 106 . 5 . 831–6 . November 1989 . 10.1093/oxfordjournals.jbchem.a122939 . 2613690 .
3. Aramaki H, Koga H, Sagara Y, Hosoi M, Horiuchi T . Complete nucleotide sequence of the 5-exo-hydroxycamphor dehydrogenase gene on the CAM plasmid of Pseudomonas putida (ATCC 17453) . Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression . 1174 . 1 . 91–4 . July 1993 . 8334169 . 10.1016/0167-4781(93)90098-x .