5-(carboxyamino)imidazole ribonucleotide mutase explained

5-(carboxyamino)imidazole ribonucleotide mutase
Ec Number:	5.4.99.18

In enzymology, a 5-(carboxyamino)imidazole ribonucleotide mutase is an enzyme that catalyzes the chemical reaction

5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole

\rightleftharpoons 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate

Hence, this enzyme has one substrate, 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole, and one product, 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.

This enzyme belongs to the family of isomerases, specifically those intramolecular transferases transferring other groups. The systematic name of this enzyme class is 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole carboxymutase. Other names in common use include N5-CAIR mutase, PurE, N5-carboxyaminoimidazole ribonucleotide mutase, and class I PurE.

References

• Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM . 1992 . Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway . Biochemistry . 31 . 5022–32 . 1534690 . 10.1021/bi00136a016 . 21 .
• Mueller EJ, Meyer E, Rudolph J, Davisson VJ, Stubbe J . 1994 . N5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli . Biochemistry . 33 . 2269–78 . 8117684 . 10.1021/bi00174a038 . 8 .
• Meyer E, Kappock TJ, Osuji C, Stubbe J . 1999 . Evidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase . Biochemistry . 38 . 3012–8 . 10074353 . 10.1021/bi9827159 . 10 .
• Mathews II, Kappock TJ, Stubbe J, Ealick SE . 1999 . Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway . Structure . 7 . 1395–406 . 10574791 . 10.1016/S0969-2126(00)80029-5 . 11 . free .
• Firestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ . 1994 . Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms . Biochemistry . 33 . 11927–34 . 7918411 . 10.1021/bi00205a031 . 39 .
• Davisson VJ . 1998 . Biochemical role of the Cryptococcus neoformans ADE2 protein in fungal de novo purine biosynthesis . Arch. Biochem. Biophys. . 351 . 123 - 34 . 9500840 . 10.1006/abbi.1997.0512 . Misialek . S . Toffaletti . DL . Klem . TJ . Perfect . JR . Davisson . VJ . 1 .