5,10-methylenetetrahydromethanopterin reductase explained

coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase
Ec Number:	1.5.98.2
Go Code:	0018537

In enzymology, a 5,10-methylenetetrahydromethanopterin reductase is an enzyme that catalyzes the chemical reaction

5-methyltetrahydromethanopterin + coenzyme F₄₂₀

\rightleftharpoons

5,10-methylenetetrahydromethanopterin + reduced coenzyme F₄₂₀

Thus, the two substrates of this enzyme are 5-methyltetrahydromethanopterin and coenzyme F₄₂₀, whereas its two products are 5,10-methylenetetrahydromethanopterin and reduced coenzyme F₄₂₀.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with other acceptors. The systematic name of this enzyme class is 5-methyltetrahydromethanopterin:coenzyme-F420 oxidoreductase. Other names in common use include 5,10-methylenetetrahydromethanopterin cyclohydrolase, N5,N10-methylenetetrahydromethanopterin reductase, methylene-H4MPT reductase, coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin, reductase, and N5,N10-methylenetetrahydromethanopterin:coenzyme-F420 oxidoreductase. This enzyme participates in folate biosynthesis.

References

Ma K, Thauer RK . 1990 . Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg) . Eur. J. Biochem. . 191 . 187 - 93 . 2379499 . 10.1111/j.1432-1033.1990.tb19109.x . 1 . free .
GD . 1991 . Purification and properties of 5,10-methylenetetrahydromethanopterin dehydrogenase and 5,10-methylenetetrahydromethanopterin reductase, two coenzyme F420-dependent enzymes, from Methanosarcina barkeri . Biochim. Biophys. Acta . 1079 . 293 - 302 . 1911853 . Geerts . WJ . Keltjens . JT . Van Der Drift . C . Vogels . GD . 3 . 10.1016/0167-4838(91)90072-8.
Ma K, Thauer RK . 1990 . Single step purification of methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum by specific binding to blue sepharose CL-6B . FEBS Lett. . 268 . 59 - 62 . 1696553 . 10.1016/0014-5793(90)80972-L . 1 . 26822476 . free . 1990FEBSL.268...59M .
Vogels GD . 1990 . Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta H . J. Biol. Chem. . 265 . 1852 - 7 . 2298726 . Hensgens . CM . Keltjens . JT . Van Der Drift . C . Vogels . GD . 4 . 10.1016/S0021-9258(19)39907-7 . free .
Drift C, Vogels GD . 1990 . Purification and properties of 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanosarcina barkeri . J. Bacteriol. . 172 . 564 - 71 . 2298699 . 2 . 10.1128/jb.172.2.564-571.1990 . 208478 .