4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase explained

4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
Ec Number:	2.7.1.148
Go Code:	0050515

In enzymology, a 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase is an enzyme that catalyzes the chemical reaction

ATP + 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate

\rightleftharpoons

ADP + 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate

Thus, the two substrates of this enzyme are ATP and 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME), whereas its two products are ADP and 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-MEP).

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol 2-phosphotransferase. This enzyme is also called CDP-ME kinase, and IspE. This enzyme participates in the MEP pathway (non-mevalonate pathway) of isoprenoid precursor biosynthesis.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes,,,,,, and .

References

Lüttgen H, Rohdich F, Herz S, Wungsintaweekul J, Hecht S, Schuhr CA, Fellermeier M, Sagner S, Zenk MH, Bacher A, Eisenreich W . 2000 . Biosynthesis of terpenoids: YchB protein of Escherichia coli phosphorylates the 2-hydroxy group of 4-diphosphocytidyl-2C-methyl-d-erythritol . Proc. Natl. Acad. Sci. U.S.A. . 97 . 1062–7 . 10655484 . 10.1073/pnas.97.3.1062 . 3 . 15522 . 2000PNAS...97.1062L . free .
Kuzuyama T, Takagi M, Kaneda K, Watanabe H, Dairi T, Seto H . 2000 . Studies on the nonmevalonate pathway: conversion of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol to its 2-phospho derivative by 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase . Tetrahedron Lett. . 41 . 2925–2928 . 10.1016/S0040-4039(00)00295-1 . 16 .