4-Fluoro-7-nitrobenzofurazan explained

4-Fluoro-7-nitrobenzofurazan (NBD-F) is a fluorogenic, amine labeling dye that is not fluorescent itself, but covalently reacts with secondary or primary amines to form a fluorescently labeled product.[1] It and other fluorogenic benzofurans (i.e. NBD-Cl) are used for derivitization in HPLC applications.[2] After the fluorogenic reaction, it can be detected with an excitation wavelength of 470 nm (blue) and an emission wavelength of 530 nm (green), enabling an HPLC limit of detection of 10 fmol.[3]

See also

Notes and References

  1. Stoyanov . Alexander V. . Ahmadzadeh . Hossein . Krylov . Sergey N. . 2002-11-25 . Heterogeneity of protein labeling with a fluorogenic reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde . Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences . 780 . 2 . 283–287 . 10.1016/s1570-0232(02)00535-4 . 1570-0232 . 12401353.
  2. Maroulis . Marios . Monemvasios . Ioannis . Vardaka . Elisabeth . Rigas . Pantelis . Determination of domoic acid in mussels by HPLC with post-column derivatization using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and fluorescence detection . Journal of Chromatography B . Elsevier BV . 876 . 2 . 2008 . 1570-0232 . 10.1016/j.jchromb.2008.10.053 . 245–251. 19026599 .
  3. Watanabe . Yoshihiko . Imai . Kazuhiro . High-performance liquid chromatography and sensitive detection of amino acids derivatized with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole . Analytical Biochemistry . Elsevier BV . 116 . 2 . 1981 . 0003-2697 . 10.1016/0003-2697(81)90390-0 . 471–472. 7316179 .

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