4-Bromoanisole Explained

4-Bromoanisole is the organobromine compound with the formula CH₃OC₆H₄Br. It is colorless liquid with a pleasant smell similar to that of anise seed. It is one of three isomers of bromoanisole, the others being 3-bromoanisole and 2-bromoanisole. It is the precursor to many 4-anisyl derivatives.

Reactions and uses

4-Bromoanisole forms a Grignard reagent,^[1] which reacts with phosphorus trichloride to give tris(4-methoxyphenyl)phosphine:

3 CH₃OC₆H₄MgBr + PCl₃ → (CH₃OC₆H₄)₃P + 3 MgBrCl4-Bromoanisole forms the organozinc derivative CH₃OC₆H₄ZnBr.^[2]

4-Bromoanisole is compound sometimes used in RNA extraction which serves to further eliminate DNA contamination.^[3] It interacts with genomic DNA (gDNA) and through a separation phase, it will be located in the organic layer instead of the aqueous layer (upper layer) containing the RNA extract.^[4]

See also

• Anisole

Notes and References

1. 10.15227/orgsyn.071.0097. 4-Methoxy-4'-Nitrobiphenyl. Organic Syntheses. 1993. 71. 97. J. K.. Stille. Antonio M. . Echavarren. Robert M.. Williams. James A.. Hendrix.
2. 10.15227/orgsyn.089.0283. Multicomponent Synthesis of Tertiary Diarylmethylamines: 1-((4-Fluorophenyl)(4-methoxyphenyl)methyl)piperidine. Organic Syntheses. 2012. 89. 283. Erwan . Le Gall. Thierry. Martens. free.
3. Web site: NAzol RT . mrcgene.com . March 2017 . September 20, 2019.
4. 10.3791/51443 . 24998448 . 4195580 . Isolation of Small Noncoding RNAs from Human Serum . Journal of Visualized Experiments . 88 . 51443 . 2014 . Khoury . Samantha . Ajuyah . Pamela . Tran . Nham .