3beta-hydroxy-5alpha-steroid dehydrogenase explained

3β-hydroxy-5α-steroid dehydrogenase
Ec Number:	1.1.1.278
Cas Number:	9015-81-0
Go Code:	0033704

In enzymology, a 3β-hydroxy-5α-steroid dehydrogenase is an enzyme that catalyzes the chemical reaction

3β-hydroxy-5α-pregnane-20-one + NADP⁺

\rightleftharpoons 5α-pregnan-3,20-dione + NADPH + H⁺

Thus, the two substrates of this enzyme are 3β-hydroxy-5α-pregnane-20-one (allopregnanolone) and NADP⁺, whereas its 3 products are 5α-pregnan-3,20-dione, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 3β-hydroxy-5α-steroid:NADP⁺ 3-oxidoreductase.

References

• Lindemann P . Luckner M . 1997 . Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata . . 46 . 507 - 513 . 10.1016/S0031-9422(97)00315-4 . 3 .
• Warneck HM, Seitz HU . 1990 . 3 beta-hydroxysteroid oxidoreductase in suspension cultures of Digitalis lanata EHRH . Z. Naturforsch. C . 45 . 963 - 72 . 2291772 . 9–10 .