3alpha(or 20beta)-hydroxysteroid dehydrogenase explained

3-alpha(or 20-beta)-hydroxysteroid dehydrogenase
Ec Number:	1.1.1.53
Cas Number:	9028-42-6
Go Code:	0047044
Width:	270

In enzymology, a 3alpha(or 20beta)-hydroxysteroid dehydrogenase is an enzyme that catalyzes the chemical reaction

androstan-3alpha,17beta-diol + NAD⁺

\rightleftharpoons 17beta-hydroxyandrostan-3-one + NADH + H⁺

Thus, the two substrates of this enzyme are androstan-3alpha,17beta-diol and NAD⁺, whereas its 3 products are 17beta-hydroxyandrostan-3-one, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 3alpha(or 20beta)-hydroxysteroid:NAD⁺ oxidoreductase. Other names in common use include cortisone reductase, (R)-20-hydroxysteroid dehydrogenase, dehydrogenase, 20beta-hydroxy steroid, Delta4-3-ketosteroid hydrogenase, 20beta-hydroxysteroid dehydrogenase, 3alpha,20beta-hydroxysteroid:NAD⁺-oxidoreductase, NADH-20beta-hydroxysteroid dehydrogenase, and 20beta-HSD. This enzyme participates in bile acid biosynthesis and c21-steroid hormone metabolism.

Structural studies

As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes,,,,, and .

References

• Edwards CA, Orr JC . 1978 . Comparison of the 3α- and 20β-Hydroxysteroid Dehydrogenase Activities of the Cortisone Reductase of Streptomyces hydrogenans . Biochemistry . 17 . 4370–6 . 718844 . 10.1021/bi00614a003 . 21 .
• Kung CC, Huang WN, Huang YC, Yeh KC . 2006 . Proteomic survey of copper-binding proteins in Arabidopsis roots by immobilized metal affinity chromatography and mass spectrometry . Proteomics . 6 . 2746–58 . 16526091 . 10.1002/pmic.200500108 . 9 . 25896917 .
• ((Lynn WS Jr)), Brown RH . 1958 . The conversion of progesterone to androgens by testes . J. Biol. Chem. . 232 . 1015–30 . 13549484 . 2 .
• Strickler RC, Covey DF, Tobias B . 1980 . Study of 3α,20β-Hydroxysteroid Dehydrogenase with an Enzyme-Generated Affinity Alkylator: Dual Enzyme Activity at a Single Active Site . Biochemistry . 19 . 4950–4 . 6936053 . 10.1021/bi00563a002 . 22 .
• Sweet F, Samant BR . 1980 . Bifunctional Enzyme Activity at the Same Active Site: Study of 3α and 20β Activity by Affinity Alkylation of 3α,20β-Hydroxysteroid Dehydrogenase with 17-(Bromoacetoxy)steroids . Biochemistry . 19 . 978–86 . 6928375 . 10.1021/bi00546a023 . 5 .