3-hexulose-6-phosphate synthase explained

3-hexulose-6-phosphate synthase
Ec Number:	4.1.2.43

3-hexulose-6-phosphate synthase (D-arabino-3-hexulose 6-phosphate formaldehyde-lyase, 3-hexulosephosphate synthase, 3-hexulose phosphate synthase, HPS) is an enzyme with systematic name D-arabino-hex-3-ulose-6-phosphate formaldehyde-lyase (D-ribulose-5-phosphate-forming).^{[1] [2] [3] [4] [5] [6] [7]} This enzyme catalyses the following chemical reaction

D-arabino-hex-3-ulose 6-phosphate

\rightleftharpoons D-ribulose 5-phosphate + formaldehyde

This enzyme requires Mg²⁺ or Mn²⁺ for maximal activity.

Notes and References

1. Ferenci T, Strom T, Quayle JR . Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus . The Biochemical Journal . 144 . 3 . 477–86 . December 1974 . 10.1042/bj1440477 . 4219834 . 1168525 .
2. Kato N, Ohashi H, Tani Y, Ogata K . 3-Hexulosephosphate synthase from Methylomonas aminofaciens 77a. Purification, properties and kinetics . Biochimica et Biophysica Acta (BBA) - Enzymology . 523 . 1 . 236–44 . March 1978 . 564713 . 10.1016/0005-2744(78)90026-8 .
3. Yanase H, Ikeyama K, Mitsui R, Ra S, Kita K, Sakai Y, Kato N . Cloning and sequence analysis of the gene encoding 3-hexulose-6-phosphate synthase from the methylotrophic bacterium, Methylomonas aminofaciens 77a, and its expression in Escherichia coli . FEMS Microbiology Letters . 135 . 2–3 . 201–5 . January 1996 . 8595859 . 10.1111/j.1574-6968.1996.tb07990.x . free .
4. Yurimoto H, Kato N, Sakai Y . Assimilation, dissimilation, and detoxification of formaldehyde, a central metabolic intermediate of methylotrophic metabolism . Chemical Record . 5 . 6 . 367–75 . 2005 . 16278835 . 10.1002/tcr.20056 .
5. Kato N, Yurimoto H, Thauer RK . The physiological role of the ribulose monophosphate pathway in bacteria and archaea . Bioscience, Biotechnology, and Biochemistry . 70 . 1 . 10–21 . January 2006 . 16428816 . 10.1271/bbb.70.10 . 21562372 .
6. Orita I, Yurimoto H, Hirai R, Kawarabayasi Y, Sakai Y, Kato N . The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathway . Journal of Bacteriology . 187 . 11 . 3636–42 . June 2005 . 15901685 . 1112069 . 10.1128/jb.187.11.3636-3642.2005 .
7. 3-Hexulose phosphate pynthase from a new facultative methylotroph, Mycobacterium gastri MB19 . Kato, N. . Miyamoto, N. . Shimao, M. . Sakazawa, C. . Agric. Biol. Chem. . 1988 . 52 . 10 . 2659–2661 . 10.1271/bbb1961.52.2659. free .