3-Ketosteroid 9alpha-monooxygenase explained

3-ketosteroid 9alpha-monooxygenase
Ec Number:1.14.13.142

3-ketosteroid 9alpha-monooxygenase (KshAB, 3-ketosteroid 9alpha-hydroxylase) is an enzyme with systematic name androsta-1,4-diene-3,17-dione,NADH:oxygen oxidoreductase (9alpha-hydroxylating).[1] [2] [3] This enzyme catalyses the following chemical reaction

androsta-1,4-diene-3,17-dione + NADH + H+ + O2

\rightleftharpoons

9alpha-hydroxyandrosta-1,4-diene-3,17-dione + NAD+ + H2O

3-Ketosteroid 9alpha-monooxygenase is involved in the cholesterol degradation in several bacterial pathogens.

Notes and References

  1. Petrusma M, Dijkhuizen L, van der Geize R . Rhodococcus rhodochrous DSM 43269 3-ketosteroid 9alpha-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate specificity . Applied and Environmental Microbiology . 75 . 16 . 5300–7 . August 2009 . 19561185 . 2725467 . 10.1128/AEM.00066-09 . 2009ApEnM..75.5300P .
  2. Capyk JK, D'Angelo I, Strynadka NC, Eltis LD . Characterization of 3-ketosteroid 9-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis . The Journal of Biological Chemistry . 284 . 15 . 9937–46 . April 2009 . 19234303 . 2665117 . 10.1074/jbc.M900719200 . free .
  3. Capyk JK, Casabon I, Gruninger R, Strynadka NC, Eltis LD . Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis . The Journal of Biological Chemistry . 286 . 47 . 40717–24 . November 2011 . 21987574 . 3220451 . 10.1074/jbc.M111.289975 . free .

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