3-Isopropylmalate dehydrogenase explained

3-Isopropylmalate dehydrogenase
Ec Number:	1.1.1.85
Cas Number:	9030-97-1
Go Code:	0003862

3-Isopropylmalate dehydrogenase is an enzyme that is a part of the isopropylmalate dehydrogenase family, which catalyzes the chemical reactions:^{[1] [2] [3] [4]}

\rightleftharpoons 4-methyl-2-oxopentanoate + CO₂ + NADH

(2R,3S)-3-isopropylmalate + NAD⁺

\rightleftharpoons (2S)-2-isopropyl-3-oxosuccinate + H⁺ + NADH

(2S)-2-isopropyl-3-oxosuccinate + H⁺

\rightleftharpoons 4-methyl-2-oxopentanoate + CO₂

Notes and References

1. Burns RO, Umbarger HE, Gross SR . The biosynthesis of leucine. III. The conversion of α-hydroxy-β-carboxyisocaproate to α-ketoisocaproate. . Biochemistry . 2 . 5. 1053–8 . 1963 . 14087358 . 10.1021/bi00905a024.
2. Calvo JM, Stevens CM, Kalyanpur MG, Umbarger HE . The Absolute Configuration of α-carboxyisocaproic Acid (3-Isopropylmalic Acid), an Intermediate in Leucine Biosynthesis . Biochemistry . 3 . 12. 2024–7 . December 1964 . 14269331 . 10.1021/bi00900a043.
3. Parsons SJ, Burns RO . Purification and Properties of β-Isopropylmalate Dehydrogenase . J. Biol. Chem. . 244 . 3 . 996–1003 . February 1969 . 4889950 .
4. Németh A, Svingor A, Pócsik M, Dobó J, Magyar C, Szilágyi A, Gál P, Závodszky P . Mirror image mutations reveal the significance of an intersubunit ion cluster in the stability of 3-isopropylmalate dehydrogenase . FEBS Lett. . 468 . 1 . 48–52 . February 2000 . 10683439 . 10.1016/S0014-5793(00)01190-X. 44544921 .