3-(3-hydroxyphenyl)propanoate hydroxylase explained

3-(3-hydroxyphenyl)propanoate hydroxylase
Ec Number:	1.14.13.127

3-(3-hydroxyphenyl)propanoate hydroxylase (mhpA (gene)) is an enzyme with systematic name 3-(3-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (2-hydroxylating).^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

(1) 3-(3-hydroxyphenyl)propanoate + NADH + H⁺ + O₂

\rightleftharpoons 3-(2,3-dihydroxyphenyl)propanoate + H₂O + NAD⁺

(2) (2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + H⁺ + O₂

\rightleftharpoons (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H₂O + NAD⁺

3-(3-hydroxyphenyl)propanoate hydroxylase is a flavoprotein (FAD).

Notes and References

1. Burlingame R, Chapman PJ . Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli . Journal of Bacteriology . 155 . 1 . 113–21 . July 1983 . 6345502 . 217659 .
2. Burlingame RP, Wyman L, Chapman PJ . Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation . Journal of Bacteriology . 168 . 1 . 55–64 . October 1986 . 3531186 . 213419 .
3. Ferrández A, Garciá JL, Díaz E . Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12 . Journal of Bacteriology . 179 . 8 . 2573–81 . April 1997 . 9098055 . 179006 .
4. Díaz E, Ferrández A, García JL . Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12 . Journal of Bacteriology . 180 . 11 . 2915–23 . June 1998 . 9603882 . 107259 .