3,5-Difluoro-4-hydroxybenzylidene imidazolinone explained

3,5-Difluoro-4-hydroxybenzylidene imidazolinone or DFHBI[1] is an imidazolinone fluorophore used in various biochemical studies. The benzene ring of DFHBI can freely rotate around the single bond but when it is fixed in planar conformation, DFHBI fluoresces.[2] It is a synthetic analog of the GFP fluorophore.

Notes and References

  1. Web site: DFHBI 1241390-29-3. live. Sigma Aldrich. https://web.archive.org/web/20220113113934/https://www.sigmaaldrich.com/CZ/en/product/sigma/sml1627 . 2022-01-13 .
  2. Dou. Jiayi. Vorobieva. Anastassia A.. Sheffler. William. Doyle. Lindsey A.. Park. Hahnbeom. Bick. Matthew J.. Mao. Binchen. Foight. Glenna W.. Lee. Min Yen. Gagnon. Lauren A.. Carter. Lauren. September 2018. De novo design of a fluorescence-activating β-barrel. Nature. en. 561. 7724. 485–491. 10.1038/s41586-018-0509-0. 0028-0836. 6275156. 30209393. 2018Natur.561..485D .

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