The 23rd General Assembly of Nova Scotia represented Nova Scotia between 1864 and 1867.
The assembly sat at the pleasure of the Lieutenant-Governor of Nova Scotia, Charles Hastings Doyle. Richard Graves MacDonnell was governor for Nova Scotia from May 1864 to October 1865. William Fenwick Williams served as governor from 1865 to 1867.
John C. Wade was chosen as speaker for the house.
The assembly was dissolved on June 10, 1867.