23S rRNA pseudouridine955/2504/2580 synthase explained

23S rRNA pseudouridine^955/2504/2580 synthase
Ec Number:	5.4.99.24

23S rRNA pseudouridine^{955/2504/2580} synthase (RluC, pseudouridine synthase RluC) is an enzyme with systematic name 23S rRNA-uridine^{955/2504/2580} uracil mutase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

23S rRNA uridine⁹⁵⁵/uridine²⁵⁰⁴/uridine²⁵⁸⁰

\rightleftharpoons 23S rRNA pseudouridine⁹⁵⁵/pseudouridine²⁵⁰⁴/pseudouridine²⁵⁸⁰

The enzyme converts uridines at position 955, 2504 and 2580 of 23S rRNA to pseudouridines.

Notes and References

1. Jiang M, Sullivan SM, Walker AK, Strahler JR, Andrews PC, Maddock JR . Identification of novel Escherichia coli ribosome-associated proteins using isobaric tags and multidimensional protein identification techniques . Journal of Bacteriology . 189 . 9 . 3434–44 . May 2007 . 17337586 . 1855874 . 10.1128/jb.00090-07 .
2. Conrad J, Sun D, Englund N, Ofengand J . The rluC gene of Escherichia coli codes for a pseudouridine synthase that is solely responsible for synthesis of pseudouridine at positions 955, 2504, and 2580 in 23 S ribosomal RNA . The Journal of Biological Chemistry . 273 . 29 . 18562–6 . July 1998 . 9660827 . 10.1074/jbc.273.29.18562 . free .
3. Corollo D, Blair-Johnson M, Conrad J, Fiedler T, Sun D, Wang L, Ofengand J, Fenna R . Crystallization and characterization of a fragment of pseudouridine synthase RluC from Escherichia coli . Acta Crystallographica Section D . 55 . Pt 1 . 302–4 . January 1999 . 10089432 . 10.1107/s090744499801021x .
4. Toh SM, Mankin AS . An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors . Journal of Molecular Biology . 380 . 4 . 593–7 . July 2008 . 18554609 . 5367387 . 10.1016/j.jmb.2008.05.027 .