23S rRNA pseudouridine746 synthase explained

23S rRNA pseudouridine^746 synthase
Ec Number:	5.4.99.29

23S rRNA pseudouridine⁷⁴⁶ synthase (RluA, 23S RNA PSI⁷⁴⁶ synthase, 23S rRNA pseudouridine synthase, pseudouridine synthase RluA) is an enzyme with systematic name 23S rRNA-uridine⁷⁴⁶ uracil mutase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

23S rRNA uridine⁷⁴⁶

\rightleftharpoons 23S rRNA pseudouridine⁷⁴⁶

RluA is the only protein responsible for the in vivo formation of 23S RNA pseudouridine⁷⁴⁶.

Notes and References

1. Hoang C, Chen J, Vizthum CA, Kandel JM, Hamilton CS, Mueller EG, Ferré-D'Amaré AR . Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure . Molecular Cell . 24 . 4 . 535–45 . November 2006 . 17188032 . 10.1016/j.molcel.2006.09.017 . free .
2. Raychaudhuri S, Niu L, Conrad J, Lane BG, Ofengand J . Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA . The Journal of Biological Chemistry . 274 . 27 . 18880–6 . July 1999 . 10383384 . 10.1074/jbc.274.27.18880 . free .
3. Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J . A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe) . RNA . 1 . 4 . 437–48 . June 1995 . 7493321 . 1482406 .