23S rRNA pseudouridine2604 synthase explained

23S rRNA pseudouridine^2604 synthase
Ec Number:	5.4.99.21

23S rRNA pseudouridine²⁶⁰⁴ synthase (RluF, YjbC) is an enzyme with systematic name 23S rRNA-uridine2604 uracil mutase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

23S rRNA uridine²⁶⁰⁴

\rightleftharpoons 23S rRNA pseudouridine²⁶⁰⁴

The enzyme can, to a small extent, also react with uridine²⁶⁰⁵.

Notes and References

1. Del Campo M, Kaya Y, Ofengand J . Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli . RNA . 7 . 11 . 1603–15 . November 2001 . 11720289 . 1370202 .
2. Alian A, DeGiovanni A, Griner SL, Finer-Moore JS, Stroud RM . Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome . Journal of Molecular Biology . 388 . 4 . 785–800 . May 2009 . 19298824 . 2796871 . 10.1016/j.jmb.2009.03.029 .
3. Sunita S, Zhenxing H, Swaathi J, Cygler M, Matte A, Sivaraman J . Domain organization and crystal structure of the catalytic domain of E.coli RluF, a pseudouridine synthase that acts on 23S rRNA . Journal of Molecular Biology . 359 . 4 . 998–1009 . June 2006 . 16712869 . 10.1016/j.jmb.2006.04.019 .