23S rRNA (uridine2552-2'-O)-methyltransferase explained

23S rRNA (uridine2552-2'-O)-methyltransferase
Ec Number:	2.1.1.166

23S rRNA (uridine²⁵⁵2-2'-O)-methyltransferase (Um(2552) 23S ribosomal RNA methyltransferase, heat shock protein RrmJ, RrmJ, FTSJ, Um²⁵⁵² methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uridine²⁵⁵²-2'-O-)-methyltransferase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + uridine²⁵⁵² in 23S rRNA

\rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methyluridine²⁵⁵² in 23S rRNA

The enzyme catalyses the 2'-O-methylation of the universally conserved U²⁵⁵² in the A loop of 23S rRNA.

Notes and References

1. Caldas T, Binet E, Bouloc P, Costa A, Desgres J, Richarme G . The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23 S ribosomal RNA methyltransferase . The Journal of Biological Chemistry . 275 . 22 . 16414–9 . June 2000 . 10748051 . 10.1074/jbc.M001854200 . free .
2. Hager J, Staker BL, Bugl H, Jakob U . Active site in RrmJ, a heat shock-induced methyltransferase . The Journal of Biological Chemistry . 277 . 44 . 41978–86 . November 2002 . 12181314 . 10.1074/jbc.M205423200 . free .
3. Hager J, Staker BL, Jakob U . Substrate binding analysis of the 23S rRNA methyltransferase RrmJ . Journal of Bacteriology . 186 . 19 . 6634–42 . October 2004 . 15375145 . 516604 . 10.1128/JB.186.19.6634-6642.2004 .
4. Bügl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U . RNA methylation under heat shock control . Molecular Cell . 6 . 2 . 349–60 . August 2000 . 10983982 . 10.1016/S1097-2765(00)00035-6 . free .