23S rRNA (uracil1939-C5)-methyltransferase explained

23S rRNA (uracil^1939-C^5)-methyltransferase
Ec Number:	2.1.1.190

23S rRNA (uracil¹⁹³⁹-C⁵)-methyltransferase (RumA, RNA uridine methyltransferase A, YgcA) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uracil¹⁹³⁹-C⁵)-methyltransferase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

\rightleftharpoons S-adenosyl-L-homocysteine + 5-methyluracil¹⁹³⁹ in 23S rRNA

The enzyme specifically methylates uracil¹⁹³⁹ at C⁵ in 23S rRNA.

Notes and References

1. Agarwalla S, Kealey JT, Santi DV, Stroud RM . Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli . The Journal of Biological Chemistry . 277 . 11 . 8835–40 . March 2002 . 11779873 . 10.1074/jbc.M111825200 . free .
2. Lee TT, Agarwalla S, Stroud RM . Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase . Structure . 12 . 3 . 397–407 . March 2004 . 15016356 . 10.1016/j.str.2004.02.009 . free .
3. Madsen CT, Mengel-Jørgensen J, Kirpekar F, Douthwaite S . Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry . Nucleic Acids Research . 31 . 16 . 4738–46 . August 2003 . 12907714 . 169892 . 10.1093/nar/gkg657 .
4. Persaud C, Lu Y, Vila-Sanjurjo A, Campbell JL, Finley J, O'Connor M . Mutagenesis of the modified bases, m(5)U1939 and psi2504, in Escherichia coli 23S rRNA . Biochemical and Biophysical Research Communications . 392 . 2 . 223–7 . February 2010 . 20067766 . 10.1016/j.bbrc.2010.01.021 .
5. Agarwalla S, Stroud RM, Gaffney BJ . Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies . The Journal of Biological Chemistry . 279 . 33 . 34123–9 . August 2004 . 15181002 . 1237038 . 10.1074/jbc.M405702200 . free .
6. Lee TT, Agarwalla S, Stroud RM . A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function . Cell . 120 . 5 . 599–611 . March 2005 . 15766524 . 10.1016/j.cell.2004.12.037 . free .