23S rRNA (guanine748-N1)-methyltransferase explained

23S rRNA (guanine^748-N^1)-methyltransferase
Ec Number:	2.1.1.188

23S rRNA (guanine⁷⁴⁸-N¹)-methyltransferase (Rlma(II), Rlma2, 23S rRNA m1G748 methyltransferase, RlmaII, Rlma II, tylosin-resistance methyltransferase RlmA(II), TlrB, rRNA large subunit methyltransferase II) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (guanine⁷⁴⁸-N¹)-methyltransferase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

\rightleftharpoons S-adenosyl-L-homocysteine + N¹-methylguanine⁷⁴⁸ in 23S rRNA

The enzyme specifically methylates guanine⁷⁴⁸ at N¹ in 23S rRNA.

Notes and References

1. Douthwaite S, Crain PF, Liu M, Poehlsgaard J . The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748 . Journal of Molecular Biology . 337 . 5 . 1073–7 . April 2004 . 15046978 . 10.1016/j.jmb.2004.02.030 .
2. Liu M, Kirpekar F, Van Wezel GP, Douthwaite S . The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA . Molecular Microbiology . 37 . 4 . 811–20 . August 2000 . 10972803 . 10.1046/j.1365-2958.2000.02046.x . 1887/3221371 . free .
3. Lebars I, Yoshizawa S, Stenholm AR, Guittet E, Douthwaite S, Fourmy D . Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII . The EMBO Journal . 22 . 2 . 183–92 . January 2003 . 12514124 . 140097 . 10.1093/emboj/cdg022 .
4. Lebars I, Husson C, Yoshizawa S, Douthwaite S, Fourmy D . Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II) . Journal of Molecular Biology . 372 . 2 . 525–34 . September 2007 . 17673230 . 10.1016/j.jmb.2007.06.068 .
5. Douthwaite S, Jakobsen L, Yoshizawa S, Fourmy D . Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I . Journal of Molecular Biology . 378 . 5 . 969–75 . May 2008 . 18406425 . 10.1016/j.jmb.2008.03.024 .
6. Liu M, Douthwaite S . Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria . Molecular Microbiology . 44 . 1 . 195–204 . April 2002 . 11967079 . 10.1046/j.1365-2958.2002.02866.x . free .