23S rRNA (guanine745-N1)-methyltransferase explained

23S rRNA (guanine^745-N^1)-methyltransferase
Ec Number:	2.1.1.187

23S rRNA (guanine⁷⁴⁵-N¹)-methyltransferase (Rlma(I), Rlma1, 23S rRNA m1G745 methyltransferase, YebH, RlmAI methyltransferase, ribosomal RNA(m1G)-methylase, rRNA(m1G)methylase, RrmA, 23S rRNA:m1G745 methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (guanine⁷⁴⁵-N¹)-methyltransferase.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

\rightleftharpoons S-adenosyl-L-homocysteine + N¹-methylguanine⁷⁴⁵ in 23S rRNA

The enzyme specifically methylates guanine⁷⁴⁵ at N¹ in 23S rRNA.

Notes and References

1. Liu M, Novotny GW, Douthwaite S . Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase . RNA . 10 . 11 . 1713–20 . November 2004 . 15388872 . 1370659 . 10.1261/rna.7820104 .
2. Gustafsson C, Persson BC . Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant . Journal of Bacteriology . 180 . 2 . 359–65 . January 1998 . 10.1128/JB.180.2.359-365.1998 . 9440525 . 106891 .
3. Das K, Acton T, Chiang Y, Shih L, Arnold E, Montelione GT . Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site . Proceedings of the National Academy of Sciences of the United States of America . 101 . 12 . 4041–6 . March 2004 . 14999102 . 384692 . 10.1073/pnas.0400189101 . 2004PNAS..101.4041D . free .
4. Hansen LH, Kirpekar F, Douthwaite S . Recognition of nucleotide G745 in 23 S ribosomal RNA by the rrmA methyltransferase . Journal of Molecular Biology . 310 . 5 . 1001–10 . July 2001 . 11501991 . 10.1006/jmbi.2001.4836 .
5. Liu M, Douthwaite S . Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria . Molecular Microbiology . 44 . 1 . 195–204 . April 2002 . 11967079 . 10.1046/j.1365-2958.2002.02866.x . free .