23S rRNA (guanine2535-N1)-methyltransferase explained

23S rRNA (guanine^2535-N^1)-methyltransferase
Ec Number:	2.1.1.209

23S rRNA (guanine²⁵³⁵-N¹)-methyltransferase (AviRa) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (guanine²⁵³5-N¹)-methyltransferase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

\rightleftharpoons S-adenosyl-L-homocysteine + N¹-methylguanine²⁵³⁵ in 23S rRNA

This is one of the methyltransferases from Streptomyces viridochromogenes . Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis.^[4]

Notes and References

1. Treede I, Jakobsen L, Kirpekar F, Vester B, Weitnauer G, Bechthold A, Douthwaite S . The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose . Molecular Microbiology . 49 . 2 . 309–18 . July 2003 . 12828631 . 10.1046/j.1365-2958.2003.03558.x . free .
2. Weitnauer G, Gaisser S, Trefzer A, Stockert S, Westrich L, Quiros LM, Mendez C, Salas JA, Bechthold A . An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tü57 . Antimicrobial Agents and Chemotherapy . 45 . 3 . 690–5 . March 2001 . 11181344 . 90357 . 10.1128/aac.45.3.690-695.2001 .
3. Mosbacher TG, Bechthold A, Schulz GE . Crystal structure of the avilamycin resistance-conferring methyltransferase AviRa from Streptomyces viridochromogenes . Journal of Molecular Biology . 329 . 1 . 147–57 . May 2003 . 12742024 . 10.1016/s0022-2836(03)00407-8 .
4. Krupkin M, Wekselman I, Matzov D, Eyal Z, Diskin Posner Y, Rozenberg H, Zimmerman E, Bashan A, Yonath A . Avilamycin and evernimicin induce structural changes in rProteins uL16 and CTC that enhance the inhibition of A-site tRNA binding . Proceedings of the National Academy of Sciences of the United States of America . 113 . 44 . E6796–E6805 . November 2016 . 27791159 . 5098648 . 10.1073/pnas.1614297113 . free .