23S rRNA (guanine1835-N2)-methyltransferase explained

23S rRNA (guanine^1835-N^2)-methyltransferase
Ec Number:	2.1.1.174

23S rRNA (guanine¹⁸³⁵-N²)-methyltransferase (ygjO (gene), rlmG (gene), ribosomal RNA large subunit methyltransferase G) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (guanine¹⁸³⁵-N²)-methyltransferase.^[1] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + guanine¹⁸³⁵ in 23S rRNA

\rightleftharpoons S-adenosyl-L-homocysteine + N²-methylguanine¹⁸³⁵ in 23S rRNA

The enzyme methylates 23S rRNA in vitro, assembled 50S subunits are not a substrate.

Notes and References

1. Sergiev PV, Lesnyak DV, Bogdanov AA, Dontsova OA . Identification of Escherichia coli m2G methyltransferases: II. The ygjO gene encodes a methyltransferase specific for G1835 of the 23 S rRNA . Journal of Molecular Biology . 364 . 1 . 26–31 . November 2006 . 17010380 . 10.1016/j.jmb.2006.09.008 .