23S rRNA (adenosine1067-2'-O)-methyltransferase explained

23S rRNA (adenosine^1067-2'-O)-methyltransferase
Ec Number:	2.1.1.230

23S rRNA (adenosine¹⁰⁶⁷-2'-O)-methyltransferase (23S rRNA A1067 2'-methyltransferase, thiostrepton-resistance methylase, nosiheptide-resistance methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenosine¹⁰⁶⁷-2'-O)-methyltransferase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + adenosine¹⁰⁶⁷ in 23S rRNA

\rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methyladenosine¹⁰⁶⁷ in 23S rRNA

The methylase that is responsible for autoimmunity in the thiostrepton producer Streptomyces azureus.

Notes and References

1. Bechthold A, Floss HG . Overexpression of the thiostrepton-resistance gene from Streptomyces azureus in Escherichia coli and characterization of recognition sites of the 23S rRNA A1067 2'-methyltransferase in the guanosine triphosphatase center of 23S ribosomal RNA . European Journal of Biochemistry . 224 . 2 . 431–7 . September 1994 . 7925357 . 10.1111/j.1432-1033.1994.00431.x . free .
2. Thompson J, Schmidt F, Cundliffe E . Site of action of a ribosomal RNA methylase conferring resistance to thiostrepton . The Journal of Biological Chemistry . 257 . 14 . 7915–7 . July 1982 . 6806287 .
3. Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton . Thompson, J. . Cundliffe, E. . J. Gen. Microbiol. . 1981 . 124 . 291–297 . 10.1099/00221287-124-2-291. free .
4. Yang H, Wang Z, Shen Y, Wang P, Jia X, Zhao L, Zhou P, Gong R, Li Z, Yang Y, Chen D, Murchie AI, Xu Y . Crystal structure of the nosiheptide-resistance methyltransferase of Streptomyces actuosus . Biochemistry . 49 . 30 . 6440–50 . August 2010 . 20550164 . 10.1021/bi1005915 .