23S rRNA (adenine2503-C8)-methyltransferase explained

23S rRNA (adenine^2503-C^8)-methyltransferase
Ec Number:	2.1.1.224

23S rRNA (adenine²⁵⁰³-C⁸)-methyltransferase (Cfr (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine²⁵⁰³-C⁸)-methyltransferase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + adenine²⁵⁰³ in 23S rRNA

\rightleftharpoons S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine²⁵⁰³ in 23S rRNA

This enzyme is a member of the 'AdoMet radical' (radical SAM) family.

Notes and References

1. Giessing AM, Jensen SS, Rasmussen A, Hansen LH, Gondela A, Long K, Vester B, Kirpekar F . Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria . RNA . 15 . 2 . 327–36 . February 2009 . 19144912 . 2648713 . 10.1261/rna.1371409 .
2. Kaminska KH, Purta E, Hansen LH, Bujnicki JM, Vester B, Long KS . Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria . Nucleic Acids Research . 38 . 5 . 1652–63 . March 2010 . 20007606 . 2836569 . 10.1093/nar/gkp1142 .
3. Yan F, LaMarre JM, Röhrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG . RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA . Journal of the American Chemical Society . 132 . 11 . 3953–64 . March 2010 . 20184321 . 2859901 . 10.1021/ja910850y .
4. Yan F, Fujimori DG . RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift . Proceedings of the National Academy of Sciences of the United States of America . 108 . 10 . 3930–4 . March 2011 . 21368151 . 3054002 . 10.1073/pnas.1017781108 . 2011PNAS..108.3930Y . free .
5. Grove TL, Benner JS, Radle MI, Ahlum JH, Landgraf BJ, Krebs C, Booker SJ . A radically different mechanism for S-adenosylmethionine-dependent methyltransferases . Science . 332 . 6029 . 604–7 . April 2011 . 21415317 . 10.1126/science.1200877 . 2011Sci...332..604G . 2214309 . free .
6. Boal AK, Grove TL, McLaughlin MI, Yennawar NH, Booker SJ, Rosenzweig AC . Structural basis for methyl transfer by a radical SAM enzyme . Science . 332 . 6033 . 1089–92 . May 2011 . 21527678 . 3506250 . 10.1126/science.1205358 . 2011Sci...332.1089B .