23S rRNA (adenine2503-C2,C8)-dimethyltransferase explained

23S rRNA (adenine^2503-C^2,C^8)-dimethyltransferase
Ec Number:	2.1.1.194
Cas Number:	39369-30-7

(Cfr, Cfr methyltransferase, Cfr rRNA methyltransferase) is an enzyme with systematic name .^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

\rightleftharpoons 2 S-adenosyl-L-homocysteine + 2,8-dimethyladenine²⁵⁰³ in 23S rRNA

This enzyme contains an [4Fe-S] cluster.

Notes and References

1. Yan F, LaMarre JM, Röhrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG . RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA . Journal of the American Chemical Society . 132 . 11 . 3953–64 . March 2010 . 20184321 . 2859901 . 10.1021/ja910850y .
2. Giessing AM, Jensen SS, Rasmussen A, Hansen LH, Gondela A, Long K, Vester B, Kirpekar F . Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria . RNA . 15 . 2 . 327–36 . February 2009 . 19144912 . 2648713 . 10.1261/rna.1371409 .
3. Kaminska KH, Purta E, Hansen LH, Bujnicki JM, Vester B, Long KS . Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria . Nucleic Acids Research . 38 . 5 . 1652–63 . March 2010 . 20007606 . 2836569 . 10.1093/nar/gkp1142 .