23S rRNA (adenine2503-C2)-methyltransferase explained

23S rRNA (adenine^2503-C^2)-methyltransferase
Ec Number:	2.1.1.192

23S rRNA (adenine²⁵⁰³-C²)-methyltransferase (RlmN, YfgB, Cfr) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine²⁵⁰³-C²)-methyltransferase.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

\rightleftharpoons ^{S-adenosyl-L-homocysteine} + L-methionine + 5'-deoxyadenosine + 2-methyladenine²⁵⁰³ in 23S rRNA

23S rRNA (adenine²⁵⁰³-C²)-methyltransferase contains an [4Fe-4S] cluster.

Notes and References

1. Toh SM, Xiong L, Bae T, Mankin AS . The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA . RNA . 14 . 1 . 98–106 . January 2008 . 18025251 . 2151032 . 10.1261/rna.814408 .
2. Yan F, LaMarre JM, Röhrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG . RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA . Journal of the American Chemical Society . 132 . 11 . 3953–64 . March 2010 . 20184321 . 2859901 . 10.1021/ja910850y .
3. Yan F, Fujimori DG . RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift . Proceedings of the National Academy of Sciences of the United States of America . 108 . 10 . 3930–4 . March 2011 . 21368151 . 3054002 . 10.1073/pnas.1017781108 . 2011PNAS..108.3930Y . free .
4. Grove TL, Benner JS, Radle MI, Ahlum JH, Landgraf BJ, Krebs C, Booker SJ . A radically different mechanism for S-adenosylmethionine-dependent methyltransferases . Science . 332 . 6029 . 604–7 . April 2011 . 21415317 . 10.1126/science.1200877 . 2011Sci...332..604G . 2214309 . free .
5. Boal AK, Grove TL, McLaughlin MI, Yennawar NH, Booker SJ, Rosenzweig AC . Structural basis for methyl transfer by a radical SAM enzyme . Science . 332 . 6033 . 1089–92 . May 2011 . 21527678 . 3506250 . 10.1126/science.1205358 . 2011Sci...332.1089B .