23S rRNA (adenine2085-N6)-dimethyltransferase explained

23S rRNA (adenine^2085-N^6)-dimethyltransferase
Ec Number:	2.1.1.184

23S rRNA (adenine²⁰⁸⁵-N⁶)-dimethyltransferase (ErmC' methyltransferase, ermC methylase, ermC 23S rRNA methyltransferase, rRNA:m⁶A methyltransferase ErmC', ErmC', rRNA methyltransferase ErmC' ) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine²⁰⁸⁵-N⁶)-dimethyltransferase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

\rightleftharpoons 2 S-adenosyl-L-homocysteine + N⁶-dimethyladenine²⁰⁸⁵ in 23S rRNA

ErmC is a methyltransferase that confers resistance to the macrolide-lincosamide-streptogramin B group of antibiotics by catalysing the methylation of 23S rRNA at adenine²⁰⁸⁵.

Notes and References

1. Zhong P, Pratt SD, Edalji RP, Walter KA, Holzman TF, Shivakumar AG, Katz L . Substrate requirements for ErmC' methyltransferase activity . Journal of Bacteriology . 177 . 15 . 4327–32 . August 1995 . 7543473 . 177180 .
2. Denoya C, Dubnau D . Mono- and dimethylating activities and kinetic studies of the ermC 23 S rRNA methyltransferase . The Journal of Biological Chemistry . 264 . 5 . 2615–24 . February 1989 . 2492520 .
3. Denoya CD, Dubnau D . Site and substrate specificity of the ermC 23S rRNA methyltransferase . Journal of Bacteriology . 169 . 8 . 3857–60 . August 1987 . 2440853 . 212483 .
4. Bussiere DE, Muchmore SW, Dealwis CG, Schluckebier G, Nienaber VL, Edalji RP, Walter KA, Ladror US, Holzman TF, Abad-Zapatero C . Crystal structure of ErmC', an rRNA methyltransferase which mediates antibiotic resistance in bacteria . Biochemistry . 37 . 20 . 7103–12 . May 1998 . 9585521 . 10.1021/bi973113c .
5. Schluckebier G, Zhong P, Stewart KD, Kavanaugh TJ, Abad-Zapatero C . The 2.2 A structure of the rRNA methyltransferase ErmC' and its complexes with cofactor and cofactor analogs: implications for the reaction mechanism . Journal of Molecular Biology . 289 . 2 . 277–91 . June 1999 . 10366505 . 10.1006/jmbi.1999.2788 .
6. Maravić G, Bujnicki JM, Feder M, Pongor S, Flögel M . Alanine-scanning mutagenesis of the predicted rRNA-binding domain of ErmC' redefines the substrate-binding site and suggests a model for protein-RNA interactions . Nucleic Acids Research . 31 . 16 . 4941–9 . August 2003 . 12907737 . 169915 . 10.1093/nar/gkg666 .