The 2024 Tennis Open Karlsruhe was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2024 ATP Challenger Tour. It took place in Karlsruhe, Germany between 1 and 7 July 2024.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Argentina | Camilo Ugo Carabelli | 100 | 1 | |
 Slovakia | Jozef Kovalík | 114 | 2 | |
 France | Pierre-Hugues Herbert | 140 | 3 | |
| Benjamin Hassan | 156 | 4 | ||
 Kazakhstan | Denis Yevseyev | 163 | 5 | |
 Bolivia, Plurination;al State of | Hugo Dellien | 173 | 6 | |
 Hungary | Zsombor Piros | 183 | 7 | |
 Belgium | Joris De Loore | 191 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main using a protected ranking:
The following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
Edas ButvilasSee main article: 2024 Tennis Open Karlsruhe – Singles.
Jozef Kovalík def. Camilo Ugo Carabelli 6–3, 7–6(7–2).See main article: 2024 Tennis Open Karlsruhe – Doubles.
Dan Added / Grégoire Jacq 6–4, 6–0.