| Date: | 24–30 June 2024 |
| Edition: | 21st |
| Surface: | Clay / Outdoor |
| Location: | Staré Splavy, Czech Republic |
| Category: | ITF Women's World Tennis Tour |
| Prize Money: | $60,000 |
| Champs: |  Tereza Valentová |
| Champd: |  Maja Chwalińska / Anastasia Dețiuc |
| Before Year: | 2022 |
The 2024 Macha Lake Open was a professional tennis tournament that was played on outdoor clay courts. It was the twenty-first leg of the tournament, which was part of the 2024 ITF Women's World Tennis Tour. It took place in Staré Splavy, Czech Republic, between 24 and 30 June 2024. [1]
See main article: 2024 Macha Lake Open – Singles.
Tereza Valentová def. Aneta Kučmová, 6–3, 7–5See main article: 2024 Macha Lake Open – Doubles.
Maja Chwalińska / Anastasia Dețiuc def. 
Feng Shuo / Sapfo Sakellaridi, 6–3, 2–6, [10–6]| Country | Player | Rank | Seed | |
|---|---|---|---|---|
 France | Amandine Hesse | 243 | 1 | |
| Poland | Maja Chwalińska | 245 | 2 | |
 Andorra | Victoria Jiménez Kasintseva | 247 | 3 | |
 Liechtenstein | Kathinka von Deichmann | 249 | 4 | |
| Isabella Shinikova | 250 | 5 | ||
 Korea, Republic of | Jang Su-jeong | 254 | 6 | |
 Spain | Guiomar Maristany | 257 | 7 | |
| Sapfo Sakellaridi | 263 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a special ranking:
The following player received entry into the singles main draw through a special exempt:
The following players received entry from the qualifying draw:
Jessie Aney Ariana Geerlings Aneta Kučmová Katrina Scott