| Date: | 18 – 24 November |
| Edition: | 3rd |
| Draw: | 32S / 16D |
| Category: | WTA 125 |
| Location: | Charleston, South Carolina, United States |
| Venue: | LTP Mount Pleasant |
| Surface: | Outdoor clay |
| Prize Money: | $115,000 |
| Champs: |  Renata Zarazúa |
| Champd: |  Nuria Brancaccio /  Leyre Romero Gormaz |
| Main Name: | LTP Charleston Pro Tennis |
| Before Year: | 2024 |
| Before Name: | Fifth Third Charleston 125 |
The 2024 Fifth Third Charleston 125 2, sponsored by the Fifth Third Bank, was a professional women's tennis tournament played on outdoor clay courts. It was the 3rd edition of the tournament and part of the 2024 WTA 125 tournaments, offering a total of $115,000 in prize money. The first edition in 2021 was held on green clay but the surface was changed to hard for the second edition staged in March 2024. It took place at LTP Mount Pleasant in Charleston, South Carolina, United States from 18 to 24 November 2024.[1] [2]
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Mexico | Renata Zarazúa | 60 | 1 | |
 United States | Alycia Parks | 112 | 2 | |
| United States | Varvara Lepchenko | 143 | 3 | |
 Hungary | Panna Udvardy | 159 | 4 | |
| Spain | Leyre Romero Gormaz | 167 | 5 | |
| United States | Louisa Chirico | 183 | 6 | |
| United States | Hanna Chang | 197 | 7 | |
| United States | Iva Jovic | 206 | 8 |
The following players received a wildcard into the singles main draw:
Usue Maitane Arconada Lauren Davis Iva Jovic Madison SiegThe following players received entry into the singles main draw through qualification:
The following player received entry as a lucky loser:
| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
 United Kingdom | Emily Appleton | United States | Carmen Corley | 215 | 1 | |
| United States | Sophie Chang | United States | Rasheeda McAdoo | 314 | 2 | |
| Italy | Nuria Brancaccio | Spain | Leyre Romero Gormaz | 356 | 3 | |
 Australia | Jaimee Fourlis | United States | Dalayna Hewitt | 367 | 4 |
The following pair received a wildcard into the doubles main draw:
Frances Pate / Chelsea SawyerSee main article: 2024 Fifth Third Charleston 125 2 – Singles.
Renata Zarazúa def. Hanna Chang 6–1, 7–6(7–4)See main article: 2024 Fifth Third Charleston 125 2 – Doubles.
Nuria Brancaccio / Leyre Romero Gormaz def. 
Kayla Cross / Liv Hovde, 7–6(8–6), 6–2
