The 2024 Challenger de Santiago was a professional tennis tournament played on clay courts. It was the 18th edition of the tournament which was part of the 2024 ATP Challenger Tour. It took place in Santiago, Chile between 11 and 17 March 2024.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Tomás Barrios Vera | 103 | 1 | ||
 Peru | Juan Pablo Varillas | 110 | 2 | |
 Argentina | Camilo Ugo Carabelli | 127 | 3 | |
| Argentina | Facundo Bagnis | 147 | 4 | |
| Argentina | Román Andrés Burruchaga | 159 | 5 | |
| Argentina | Juan Manuel Cerúndolo | 160 | 6 | |
 Bolivia, Plurination;al State of | Hugo Dellien | 161 | 7 | |
| Argentina | Genaro Alberto Olivieri | 177 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
Valerio Aboian
Gabi Adrian Boitan
Gerard Campana Lee Federico Agustín Gómez Mariano Kestelboim
Orlando LuzSee main article: 2024 Challenger de Santiago – Singles.
Juan Pablo Varillas def. Facundo Bagnis 6–3, 6–2.See main article: 2024 Challenger de Santiago – Doubles.
Fernando Romboli / Marcelo Zormann def. Boris Arias / Federico Zeballos 7–6(7–5), 6–4.