| Season: | 2024–25 |
| Nextseason: | 2025–26 |
The 2024–25 Serbian First League is the 20th season of the Serbian First League since its establishment.
The league consist of 16 teams: ten teams from the previous season, two teams relegated from 2023–24 Serbian SuperLiga and four new teams promoted from Serbian League. Each team will play each other twice in round-robin format after which top half will play in Promotion round and bottom half in Relegation round Play-offs. First two teams from the Promotion round will be promoted to next season of Serbian Superliga, while third and fourth team will play in a Relegation Play-off. Last four teams from Relegation round will be relegated.
| Team | City | Stadium | Capacity | |
|---|---|---|---|---|
| Borac Čačak | Užice | Radomir Antić Stadium | 12,000 | |
| Dubočica | Leskovac | Dubočica Stadium | 8,136 | |
| Grafičar | Belgrade | Stadion Rajko Mitić - Jug veštačka trava | 1,000 | |
| Inđija | Inđija | Toyo Tires Arena | 4,500 | |
| Javor Ivanjica | Ivanjica | Javor Stadium | 3,000 | |
| Mačva Šabac | Šabac | Stadion FK Mačva Šabac | 5,494 | |
| Mladost Novi Sad | Novi Sad | GAT Arena | 1,400 | |
| Radnik Surdulica | Surdulica | Surdulica City Stadium | 3,312 | |
| Radnički Sremska Mitrovica | Sremska Mitrovica | Gradski stadion | 2,000 | |
| Sloboda | Užice | Radomir Antić Stadium | 12,000 | |
| Sloven Ruma | Ruma | Stadion Hesna | 2,000 | |
| Smederevo 1924 | Smederevo | Smederevo Stadium | 17,200 | |
| Trayal Kruševac | Kruševac | Mladost Stadium | 10,331 | |
| Voždovac | Voždovac | Voždovac Stadium | 5,175 | |
| Vršac | Vršac | Gradski stadion Vršac | 5,000 | |
| Zemun | Zemun | Zemun Stadium | 9,588 |