See main article: 2023 World Para Swimming Championships. The women's 50m backstroke events at the 2023 World Para Swimming Championships were held at the Manchester Aquatics Centre between 31 July and 6 August.
| S2 | ||||
| S3[1] | ||||
| S4 | ||||
| S5 |
The final took place on 3 August.
| Rank | Heat | Lane | Name | Nation | Result | Notes | |
|---|---|---|---|---|---|---|---|
| 1 | 4 | 54.9 | |||||
| 1 | 5 | 1:01.38 | |||||
| 1 | 6 | 1:01.81 | |||||
| 4 | 1 | 3 | 1:04.26 | ||||
| 5 | 1 | 2 | 1:07.82 | ||||
| 6 | 1 | 7 | 1:14.53 | ||||
| 7 | 1 | 8 | 1:15.06 | ||||
| 8 | 1 | 1 | 1:16.13 |