| Date: | 17–23 April |
| Edition: | 23rd |
| Surface: | Green clay |
| Location: | Tallahassee, Florida, United States |
| Champs: |  Zizou Bergs |
| Champd: |  Federico Agustín Gómez / Nicolás Kicker |
The 2023 Tallahassee Tennis Challenger was a professional tennis tournament played on green clay courts. It was the 23rd edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Tallahassee, Florida, United States between April 17 and April 23, 2023.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 China | Zhang Zhizhen | 95 | 1 | |
| Argentina | Camilo Ugo Carabelli | 131 | 2 | |
| Dominik Koepfer | 154 | 3 | ||
| Argentina | Facundo Díaz Acosta | 158 | 4 | |
| Wu Tung-lin | 164 | 5 | ||
| Belgium | Zizou Bergs | 165 | 6 | |
 France | Enzo Couacaud | 183 | 7 | |
 Japan | Rio Noguchi | 208 | 8 |
The following players received wildcards into the singles main draw:
Kyle Kang Bruno Kuzuhara Thai-Son KwiatkowskiThe following players received entry into the singles main draw using protected rankings:
The following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
See main article: 2023 Tallahassee Tennis Challenger – Singles.
Zizou Bergs def. Wu Tung-lin 7–5, 6–2.See main article: 2023 Tallahassee Tennis Challenger – Doubles.
Federico Agustín Gómez / Nicolás Kicker def. William Blumberg / 
Luis David Martínez 7–6(7–2), 4–6, [13–11].