| Date: | 11–17 September |
| Edition: | 3rd |
| Surface: | Clay |
| Location: | Santa Cruz de la Sierra, Bolivia |
| Champs: |  Mariano Navone |
| Champd: |  Boris Arias / Federico Zeballos |
| Before Name: | Santa Cruz Challenger II |
The 2023 Santa Cruz Challenger was a professional tennis tournament played on clay courts. It was the third edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Santa Cruz de la Sierra, Bolivia between 11 and 17 September 2023.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Argentina | Juan Manuel Cerúndolo | 93 | 1 | |
| Argentina | Genaro Alberto Olivieri | 140 | 2 | |
| Bolivia, Plurination;al State of | Hugo Dellien | 147 | 3 | |
| Argentina | Thiago Agustín Tirante | 150 | 4 | |
| Argentina | Francisco Comesaña | 160 | 5 | |
| Argentina | Mariano Navone | 184 | 6 | |
 Italy | Luciano Darderi | 185 | 7 | |
| Argentina | Camilo Ugo Carabelli | 202 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following players received entry from the qualifying draw:
See main article: 2023 Santa Cruz Challenger – Singles.
Mariano Navone def. Francisco Comesaña 4–6, 7–5, 6–1.See main article: 2023 Santa Cruz Challenger – Doubles.
Boris Arias / Federico Zeballos def. Matías Soto / Gonzalo Villanueva 6–2, 4–6, [10–7].