| Date: | 31 July – 6 August |
| Edition: | 28th (men) 26th (women) |
| Surface: | Hard / Outdoor |
| Location: | Lexington, Kentucky, United States |
| Category: | ATP Challenger Tour ITF Women's World Tennis Tour |
| Champms: |  Steve Johnson |
| Champmd: | Eliot Spizzirri / Tyler Zink |
| Champws: |  Renata Zarazúa |
| Champwd: | Alexis Blokhina / Ava Markham |
The 2023 Lexington Challenger was a professional tennis tournament played on outdoor hard courts. It was the 28th edition of the tournament which was part of the 2023 ATP Challenger Tour, and the 26th edition of the tournament which was part of the 2023 ITF Women's World Tennis Tour. It took place in Lexington, Kentucky, United States between 31 July and 6 August 2023.
See main article: 2023 Lexington Challenger – Men's singles.
Steve Johnson def. 
Arthur Cazaux 7–6(7–5), 6–4.See main article: 2023 Lexington Challenger – Men's doubles.
Eliot Spizzirri / Tyler Zink def. George Goldhoff / Vasil Kirkov 4–6, 6–3, [10–8].See main article: 2023 Lexington Challenger – Women's singles.
Renata Zarazúa def. Caroline Dolehide 1–6, 7–6(7–4), 7–5.See main article: 2023 Lexington Challenger – Women's doubles.
Alexis Blokhina / Ava Markham def. 
Olivia Gadecki / Dalayna Hewitt 6–4, 7–6(7–1).| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| France | Arthur Cazaux | 141 | 1 | |
| United States | Steve Johnson | 190 | 2 | |
| Wu Tung-lin | 195 | 3 | ||
 Korea, Republic of | Hong Seong-chan | 206 | 4 | |
| United States | Tennys Sandgren | 214 | 5 | |
| Australia | Dane Sweeny | 230 | 6 | |
| Australia | Adam Walton | 247 | 7 | |
| Australia | Tristan Schoolkate | 264 | 8 |
The following players received a wildcard into the singles main draw:
Martin Damm Cannon Kingsley
Joshua LapadatThe following player received entry into the singles main draw using a protected ranking:
The following players received entry into the singles main draw as alternates:
Ulises Blanch Nathan PonwithThe following players received entry from the qualifying draw:
Justin Boulais Tristan Boyer Stefan Dostanic Aidan Mayo
Yuki Mochizuki Philip Sekulic| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| United States | Caroline Dolehide | 102 | 1 | |
| United States | Madison Brengle | 103 | 2 | |
| Australia | Kimberly Birrell | 111 | 3 | |
| United States | Katie Volynets | 126 | 4 | |
| Australia | Olivia Gadecki | 144 | 5 | |
 United Kingdom | Katie Swan | 148 | 6 | |
| Japan | Mai Hontama | 179 | 7 | |
 China | Wang Yafan | 182 | 8 |
The following players received a wildcard into the singles main draw:
Elizabeth Eades Dalayna Hewitt McCartney Kessler Madison SiegThe following players received entry from the qualifying draw:
Chloe Beck DJ Bennett Samantha Crawford Sara Daavettila Tatum Evans Victoria Hu Grace Min Christina Rosca