| Date: | 6–12 November |
| Edition: | 19th |
| Surface: | Hard (Indoor) |
| Location: | Knoxville, United States |
| Champs: |  Alex Michelsen |
| Champd: | Cannon Kingsley /  Luis David Martínez |
The 2023 Knoxville Challenger was a professional tennis tournament played on indoor hard courts. It was the 19th edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Knoxville, United States between 6 and 12 November 2023.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| United States | Michael Mmoh | 101 | 1 | |
| Aleksandar Kovacevic | 109 | 2 | ||
| Alex Michelsen | 112 | 3 | ||
| Zachary Svajda | 143 | 4 | ||
| Emilio Nava | 148 | 5 | ||
| Titouan Droguet | 166 | 6 | ||
 Australia | ||||
 China | Shang Juncheng | 176 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw as a special exempt:
Aidan MayoThe following players received entry from the qualifying draw:
Murphy Cassone Cannon Kingsley Thai-Son KwiatkowskiThe following players received entry as lucky losers:
See main article: 2023 Knoxville Challenger – Singles.
Alex Michelsen def. Denis Kudla 7–5, 4–6, 6–2.See main article: 2023 Knoxville Challenger – Doubles.
Cannon Kingsley / Luis David Martínez def. Mac Kiger / Mitchell Krueger 7–6(7–3), 6–3.
