The 2023 Challenger de Santiago was a professional tennis tournament played on clay courts. It was the 17th edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Santiago, Chile between 6 and 12 March 2023.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Italy | Marco Cecchinato | 91 | 2 | |
 Argentina | Facundo Bagnis | 94 | 3 | |
 Bolivia, Plurination;al State of | Hugo Dellien | 96 | 4 | |
| Argentina | Juan Manuel Cerúndolo | 108 | 5 | |
| Argentina | Camilo Ugo Carabelli | 122 | 6 | |
| Italy | Franco Agamenone | 164 | 7 | |
| Argentina | Facundo Díaz Acosta | 171 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Daniel Dutra da Silva Federico Gaio
Hugo Gaston
Álvaro López San Martín Thiago Seyboth Wild Juan Bautista TorresThe following players received entry as lucky losers:
See main article: 2023 Challenger de Santiago – Singles.
Hugo Dellien def. Thiago Seyboth Wild 3–6, 6–3, 6–3.See main article: 2023 Challenger de Santiago – Doubles.
Pedro Boscardin Dias / João Lucas Reis da Silva def. 
Diego Hidalgo / 
Cristian Rodríguez 6–4, 3–6, [10–7].