The 2022 Zagreb Open was a professional tennis tournament played on clay courts. It was part of the 2022 ATP Challenger Tour. It took place in Zagreb, Croatia between 9 and 14 May 2022.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Australia | Jordan Thompson | 87 | 1 | |
| Australia | Aleksandar Vukic | 128 | 2 | |
| Australia | Christopher O'Connell | 129 | 3 | |
 Bosnia and Herzegovina | ||||
 Czech Republic | Vít Kopřiva | 167 | 5 | |
| Australia | Jason Kubler | 173 | 6 | |
 Argentina | Juan Pablo Ficovich | 182 | 7 | |
 Italy | Federico Gaio | 183 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
See main article: 2022 Zagreb Open – Singles.
Filip Misolic def. Mili Poljičak 6–3, 7–6(8–6).See main article: 2022 Zagreb Open – Doubles.
Adam Pavlásek / 
Igor Zelenay def. Domagoj Bilješko / Andrey Chepelev 4–6, 6–3, [10–2].
