| Date: | 21–27 March |
| Edition: | 2nd |
| Surface: | Clay |
| Location: | Santa Cruz de la Sierra, Bolivia |
| Champs: |  Paul Jubb |
| Champd: | Jesper de Jong / Bart Stevens |
| Before Year: | 2022 |
| Before Name: | Santa Cruz Challenger |
| Main Name: | Santa Cruz Challenger |
| After Name: | Santa Cruz Challenger |
The 2022 Santa Cruz Challenger II, also known as Dove MEN + CARE Bolivia Open[1] to differentiate it from the previous edition played the same year, was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2022 ATP Challenger Tour. It took place in Santa Cruz de la Sierra, Bolivia between 21 and 27 March 2022.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Bolivia, Plurination;al State of | ||||
| Pablo Cuevas | 104 | 2 | ||
 Peru | Juan Pablo Varillas | 117 | 3 | |
 Argentina | ||||
| Tomás Barrios Vera | 145 | 5 | ||
| Argentina | ||||
| Argentina | Facundo Mena | 183 | 7 | |
| Jesper de Jong | 189 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
Nicolás Barrientos
Corentin Denolly
Gilbert Klier Júnior
Naoki Nakagawa
Yshai Oliel Juan Bautista TorresThe following players received entry as lucky losers:
Daniel Dutra da Silva Facundo Juárez Gonzalo VillanuevaSee main article: 2022 Santa Cruz Challenger II – Singles.
Paul Jubb def. Juan Pablo Varillas 6–3, 7–6(7–5).See main article: 2022 Santa Cruz Challenger II – Doubles.
Nicolás Barrientos / 
Miguel Ángel Reyes-Varela 6–4, 3–6, [10–6].