| Date: | 24–30 January |
| Edition: | 1st |
| Surface: | Clay |
| Location: | Santa Cruz de la Sierra, Bolivia |
| Champs: |  Francisco Cerúndolo |
| Champd: |  Diego Hidalgo /  Cristian Rodríguez |
| After Year: | 2022 |
| After Name: | Santa Cruz Challenger II |
The 2022 Santa Cruz Challenger, also known as Dove MEN + CARE Challenger Bolivia,[1] was a professional tennis tournament played on clay courts. It was the first edition of the tournament which was part of the 2022 ATP Challenger Tour. It took place in Santa Cruz de la Sierra, Bolivia between 24 and 30 January 2022.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Bolivia, Plurination;al State of | Hugo Dellien | 114 | 1 | |
 Slovakia | Andrej Martin | 116 | 2 | |
| Argentina | Francisco Cerúndolo | 127 | 3 | |
| Argentina | Tomás Martín Etcheverry | 131 | 4 | |
 Czech Republic | Zdeněk Kolář | 143 | 5 | |
 Spain | Fernando Verdasco | 170 | 6 | |
| Argentina | Facundo Mena | 209 | 7 | |
| Argentina | Camilo Ugo Carabelli | 215 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
Pedro Cachin Matías Franco Descotte Diego Hidalgo Juan Carlos Prado Ángelo Cristian Rodríguez Gonzalo VillanuevaThe following player received entry as a lucky loser:
See main article: 2022 Santa Cruz Challenger – Singles.
Francisco Cerúndolo def. Camilo Ugo Carabelli 6–4, 6–3.See main article: 2022 Santa Cruz Challenger – Doubles.
Diego Hidalgo / Cristian Rodríguez def. Andrej Martin / 
Tristan-Samuel Weissborn 4–6, 6–3, [10–8].