| Date: | 31 October – 6 November |
| Edition: | 18th |
| Surface: | Clay |
| Location: | Guayaquil, Ecuador |
| Champs: | Daniel Altmaier |
| Champd: |  Guido Andreozzi / Guillermo Durán |
The 2022 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the 18th edition of the tournament which was part of the 2022 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 31 October and 6 November 2022.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Argentina | Federico Coria | 73 | 1 | |
| Argentina | Tomás Martín Etcheverry | 89 | 2 | |
| Daniel Altmaier | 106 | 3 | ||
 Peru | Juan Pablo Varillas | 121 | 4 | |
| Argentina | Juan Manuel Cerúndolo | 136 | 5 | |
 Italy | Franco Agamenone | 159 | 6 | |
| Argentina | Santiago Rodríguez Taverna | 162 | 7 | |
 Brazil | Felipe Meligeni Alves | 169 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
Blaise Bicknell Marco Cecchinato
Jan Choinski Juan Ignacio Galarza Eduardo Ribeiro Thiago Seyboth WildThe following player received entry as a lucky loser:
See main article: 2022 Challenger Ciudad de Guayaquil – Singles.
Federico Coria 6–2, 6–4.See main article: 2022 Challenger Ciudad de Guayaquil – Doubles.
Guido Andreozzi / Guillermo Durán def. Facundo Díaz Acosta / 
Luis David Martínez 6–0, 6–4.
