| Champ: |  Ashleigh Barty |
| Runner: |  Garbiñe Muguruza |
| Score: | 7–6(7–3), 6–4 |
| Draw: | 54 |
| Seeds: | 16 |
| Edition: | 1st |
| Type: | singles doubles |
| Main Name: | Australian Open Series |
See main article: 2021 Yarra Valley Classic.
The Yarra Valley Classic is a new addition to the WTA Tour in 2021.
Ashleigh Barty won the title, defeating Garbiñe Muguruza in the final, 7–6(7–3), 6–4.
Due to a delayed schedule because of a COVID-19 case at a tournament quarantine hotel, all matches from the quarterfinal stage forward played a match tiebreaker in the final set (first to ten points, win by two).
The top ten seeds received a bye into the second round.
See also: 1 and 1. Ashleigh Barty (champion)
See also: 2 and 4. 
Sofia Kenin (quarterfinals)
See also: 3 and 2. 
Karolína Plíšková (third round)
See also: 4 and 3. Petra Kvitová (third round)
See also: 5 and 2. Serena Williams (semifinals, withdrew because of right shoulder injury)
See also: 6 and 4. Garbiñe Muguruza (final)
See also: 7 and 1. Petra Martić (third round)
See also: 8 and 3. Markéta Vondroušová (semifinals)
See also: 9 and 2. Donna Vekić (second round)
See also: 10. 
Zhang Shuai (withdrew)
See also: 11 and 4. 
Anastasia Pavlyuchenkova (third round)
See also: 12 and 1. 
Fiona Ferro (first round)
See also: 13 and 2. Danielle Collins (quarterfinals)
See also: 14 and 3. 
Nadia Podoroska (quarterfinals)
See also: 15 and 4. Kristina Mladenovic (first round)
See also: 16 and 1. Marie Bouzková (third round)