| Date: | 13–19 September |
| Edition: | 22nd |
| Surface: | Clay |
| Location: | Quito, Ecuador |
| Champs: |  Facundo Mena |
| Champd: |  Alejandro Gómez / Thiago Agustín Tirante |
| Before Year: | 2017 |
The 2021 Quito Challenger was a professional tennis tournament played on clay courts. It was the 22nd edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Quito, Ecuador between 13 and 19 September 2021.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Slovakia | Andrej Martin | 122 | 1 | |
 Ecuador | Emilio Gómez | 161 | 2 | |
 Spain | Mario Vilella Martínez | 170 | 3 | |
| Argentina | Renzo Olivo | 215 | 4 | |
| Argentina | Juan Pablo Ficovich | 254 | 5 | |
| Argentina | Facundo Mena | 262 | 6 | |
| Argentina | Thiago Agustín Tirante | 268 | 7 | |
| Ecuador | Roberto Quiroz | 294 | 8 |
The following players received wildcards into the singles main draw:
Álvaro Guillén Meza Antonio Cayetano March Gian Carlos RodríguezThe following players received entry from the qualifying draw:
Alexis Gautier Alejandro Gómez
Christian LangmoSee main article: 2021 Quito Challenger – Singles.
Facundo Mena def. Gonzalo Lama 6–4, 6–4.See main article: 2021 Quito Challenger – Doubles.
Alejandro Gómez / Thiago Agustín Tirante def. Adrián Menéndez Maceiras / Mario Vilella Martínez 7–5, 6–7(5–7), [10–8].