| Date: | 12–18 April |
| Edition: | 3rd |
| Surface: | Hard |
| Location: | Orlando, United States |
| Champs: |  Jenson Brooksby |
| Champd: | Mitchell Krueger / Jack Sock |
| After Name: | Orlando Open II |
| After Year: | 2021 |
The 2021 Orlando Open was a professional tennis tournament played on hard courts. It was the third edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Orlando, United States between 12 and 18 April 2021.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Steve Johnson | 83 | 1 | ||
 Japan | Yasutaka Uchiyama | 111 | 2 | |
| Mackenzie McDonald | 119 | 3 | ||
 Brazil | Thiago Seyboth Wild | 122 | 4 | |
| Denis Kudla | 123 | 5 | ||
 India | Prajnesh Gunneswaran | 139 | 6 | |
| Jason Jung | 148 | 7 | ||
| Alejandro Tabilo | 163 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
See main article: 2021 Orlando Open – Singles.
Jenson Brooksby def. Denis Kudla 6–3, 6–3.See main article: 2021 Orlando Open – Doubles.
Mitchell Krueger / Jack Sock def. Christian Harrison / Dennis Novikov 4–6, 7–5, [13–11].
