| Date: | 25–31 October |
| Edition: | 16th |
| Surface: | Clay |
| Location: | Lima, Peru |
| Champs: | Nicolás Jarry |
| Champd: |  Sergio Galdós / Gonçalo Oliveira |
| Before Year: | 2021 |
| Before Name: | Lima Challenger |
| Main Name: | Lima Challenger |
| After Name: | Lima Challenger |
The 2021 Lima Challenger II was a professional tennis tournament played on clay courts. It was the sixteenth edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Lima, Peru between 25 and 31 October 2021.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Brazil | ||||
 Argentina | Juan Manuel Cerúndolo | 102 | 2 | |
 Colombia | Daniel Elahi Galán | 107 | 3 | |
| Argentina | ||||
| Argentina | ||||
| Brazil | Thiago Seyboth Wild | 126 | 6 | |
 Bolivia, Plurination;al State of | Hugo Dellien | 128 | 7 | |
| Peru | Juan Pablo Varillas | 129 | 8 | |
| Argentina | Tomás Martín Etcheverry | 138 | 9 | |
| Marcelo Tomás Barrios Vera | 145 | 10 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
Alexis Galarneau Cristian Rodríguez
Jean ThirouinSee main article: article and 2021 Lima Challenger II – Singles.
Juan Manuel Cerúndolo 6–2, 7–5.See main article: article and 2021 Lima Challenger II – Doubles.
Sergio Galdós / Gonçalo Oliveira def. Marcelo Tomás Barrios Vera / Alejandro Tabilo 6–2, 2–6, [10–5].
