| Champ: |  Garbiñe Muguruza |
| Runner: |  Barbora Krejčíková |
| Score: | 7–6(8–6), 6–3 |
| Draw: | 56 (8 / 3) |
| Seeds: | 16 |
See main article: 2021 Dubai Tennis Championships.
Garbiñe Muguruza defeated Barbora Krejčíková in the final, 7–6(8–6), 6–3 to win the women's singles tennis event at the 2021 Dubai Tennis Championships. Muguruza dropped just one set during the tournament, to Aryna Sabalenka in the quarterfinals.
Simona Halep was the reigning champion,[1] but withdrew before the tournament due to an injury.[2]
The top eight seeds received a bye into the second round.
See also: 1 and 1. 
Elina Svitolina (second round)
See also: 2 and 4. Karolína Plíšková (third round)
See also: 3 and 3. 
Aryna Sabalenka (quarterfinals)
See also: 4 and 2. Petra Kvitová (second round, retired)
See also: 5 and 2. Kiki Bertens (second round)
See also: 6 and 1. Belinda Bencic (third round)
See also: 7. Victoria Azarenka (withdrew)
See also: 8 and 3. 
Iga Świątek (third round)
See also: 9 and 3. Garbiñe Muguruza (champion)
See also: 10 and 4. 
Elise Mertens (semifinals)
See also: 11 and 1. 
Madison Keys (second round)
See also: 12 and 2. Markéta Vondroušová (second round)
See also: 13 and 4. Petra Martić (first round)
See also: 14 and 2. 
Elena Rybakina (second round)
See also: 15 and 3. 
Anett Kontaveit (third round)
See also: 16 and 1. Maria Sakkari (first round)