| Date: | 15–21 February |
| Edition: | 1st |
| Surface: | Clay |
| Location: | Concepción, Chile |
| Champs: |  Sebastián Báez |
| Champd: |  Orlando Luz / Rafael Matos |
The 2021 Challenger Concepción was a professional tennis tournament played on clay courts. It was the first edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Concepción, Chile between 15 and 21 February 2021.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Argentina | Federico Coria | 92 | 1 | |
 Slovakia | Andrej Martin | 103 | 2 | |
 Bolivia, Plurination;al State of | Hugo Dellien | 112 | 4 | |
 Colombia | Daniel Elahi Galán | 115 | 5 | |
| Brazil | Thiago Seyboth Wild | 117 | 6 | |
| Argentina | Facundo Bagnis | 127 | 7 | |
| Daniel Altmaier | 134 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
See main article: 2021 Challenger Concepción – Singles.
Sebastián Báez def. Francisco Cerúndolo 6–3, 6–7(5–7), 7–6(7–5).See main article: 2021 Challenger Concepción – Doubles.
Orlando Luz / Rafael Matos def. 
Sergio Galdós / 
Diego Hidalgo 7–5, 6–4.
