The 2021 Biella Challenger VII was a professional tennis tournament played on outdoor red clay courts. It was part of the 2021 ATP Challenger Tour. It took in Biella, Italy between 31 May and 6 June 2021.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Peru | Juan Pablo Varillas | 130 | 1 | |
 Serbia | Nikola Milojević | 140 | 2 | |
| Daniel Altmaier | 145 | 3 | ||
| Serbia | Danilo Petrović | 165 | 4 | |
| Frederico Ferreira Silva | 168 | 5 | ||
 Poland | Kacper Żuk | 173 | 6 | |
 Slovakia | Martin Kližan | 176 | 7 | |
 Italy | Lorenzo Giustino | 178 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as special exempts:
The following players received entry from the qualifying draw:
See main article: 2021 Biella Challenger VII – Singles.
Marco Trungelliti 6–3, 5–7, 7–6(7–5).See main article: 2021 Biella Challenger VII – Doubles.
Tomás Martín Etcheverry / Renzo Olivo def. 
Luis David Martínez / 
David Vega Hernández 3–6, 6–3, [10–8].